@ARTICLE{Khan2026-nu,
  title     = "Retrieving novel aquatic bacteria from freshwater habitats:
               preliminary work report based on nutritionally poor media",
  author    = "Khan, Biswajit and Krishnamurthi, Srinivasan and Saha, Pradipta",
  abstract  = "In an attempt to cultivate and identify oligophilic bacteria,
               295 bacterial strains were isolated from 8 different fresh water
               samples collected from different water bodies, located in
               Burdwan, Jhargram, Bankura and Purulia (in West Bengal, India).
               Among these 58 strains were oligophilic and could only grow on
               nutritionally poor media (TSBA/100 and R2A Agar), while, the
               rest belonged to facultatively oligophilic category and could
               grow on both nutritionally poor and rich (TSBA) media. Among 58
               oligophilic strains, 6 were lost during sub-culturing process.
               These oligophilic strains do not grow on most of the
               conventional culture media like Nutrient Agar (NA), Luria
               Bertani agar (LB agar), Mueller Hinton agar (MHA), MacConkey
               Agar (MAC) and on media recommended for phenotypic
               characterization. Preliminary identification of 32 oligophilic
               strains (out of 58) was carried out by using 16S rRNA gene based
               molecular phylogenetic approach. Their closest phylogenetic
               relatives were recorded to belong to 25 different genera and 32
               different species. On the basis of recent cut off limit, 10
               strains were concluded as possible putative novel taxa and were
               recorded to possess identity value less than 98.65-99\% (current
               threshold limit for delineation of prokaryotic species) to their
               nearest 16S rRNA gene sequence of representative type strains.
               Oligophilic bacteria represent component of aboriginal
               microflora and their study is important from ecological aspects.
               Although, the present study was executed with a small sample
               size and less number of isolates, nevertheless, presence of high
               beta diversity indicated that study of oligophilic bacteria from
               aquatic habitats provides an opportunity to isolate possible
               putative novel taxa which may be further taken up for detailed
               taxonomic, genomics and function based biotechnological studies.
               Supplementary Information: The online version contains
               supplementary material available at 10.1007/s13205-026-04780-5.",
  journal   = "3 Biotech",
  publisher = "Springer Science and Business Media LLC",
  volume    =  16,
  number    =  5,
  pages     = "167",
  month     =  may,
  year      =  2026,
  keywords  = "16S rRNA gene; Biotechnology; Culturomics; Nutritionally poor
               media; Oligophilic bacteria",
  copyright = "https://www.springernature.com/gp/researchers/text-and-data-mining",
  language  = "en"
}