@ARTICLE{Chouhan2026-om,
  title     = "Arl8b inactivates the Rab11a recycling pathway to promote
               {LAMP1} sorting and lysosome biogenesis",
  author    = "Chouhan, Priya and Phogat, Yogita and Walia, Kshitiz and
               Debnath, Saikat and Choubey, Sandeep and Gupta, Medha and Tuli,
               Amit and Sharma, Mahak",
  abstract  = "The small GTP-binding protein Arl8b is established as a
               regulator of lysosome positioning and fusion, yet its role in
               lysosome biogenesis remains unclear. Here, we investigate the
               role of Arl8b in the trafficking of newly synthesized LAMP1 to
               lysosomes using the Retention Using Selective Hook (RUSH) assay.
               We find that Arl8b localizes to post-endocytic LAMP1-containing
               vesicles prior to fusion with acidic lysosomes. Arl8b depletion
               leads to Rab11a-dependent recycling of LAMP1 to the plasma
               membrane, impairing its lysosomal delivery. Mechanistically,
               Arl8b recruits the Rab11a GAP, TBC1D9B, to LAMP1-positive
               membranes, and TBC1D9B depletion similarly disrupts LAMP1
               sorting. Notably, TBC1D9B knockdown also impairs the retrieval
               of cation-independent mannose-6-phosphate receptor (CI-M6PR)
               from Rab11a- and Rab14-positive endosomes to the trans-Golgi
               network, impairing pro-cathepsin trafficking and cargo
               degradation. These findings reveal that Arl8b-mediated
               recruitment of Rab GAP TBC1D9B is crucial for inactivation of
               the Rab11a recycling pathway, leading to efficient sorting of
               lysosomal cargo to their functional location.",
  journal   = "J. Cell Biol.",
  publisher = "Rockefeller University Press",
  volume    =  225,
  number    =  7,
  month     =  jul,
  year      =  2026,
  copyright = "https://creativecommons.org/licenses/by/4.0/",
  language  = "en"
}