@ARTICLE{Upadhayay2026-la,
  title     = "Unravelling chaperone-mediated microautophagy targeting
               {KFERQ-like} motif containing proteins in yeast",
  author    = "Upadhayay, Krishna and Bhardwaj, Pushpender and Farooqi, Namra
               and Rabha, Bhagyashree and Mishra, Adarsh and Verma, Kamalesh
               and Singh, Ramandeep and Sharma, Deepak",
  abstract  = "Under prolonged starvation, mammalian cells activate
               chaperone-mediated autophagy (CMA) that degrades cellular
               proteins containing KFERQ-like motifs via lysosomes. During CMA,
               the lysosomal membrane protein LAMP2A acts as an essential
               receptor for the HSPA8/HSC70-CMA substrate complex. Thus, the
               evidence of CMA in organisms lacking LAMP2A on
               lysosomes/vacuoles is still lacking. Here, we examined the fate
               of proteins containing such motifs in S. cerevisiae that lack
               the CMA receptor on vacuoles. Intriguingly, we found that even
               in the absence of LAMP2A, proteins containing such motifs
               translocate into vacuoles upon prolonged starvation. We report
               for the first time that phosphatidylserine acts as an
               Hsp70-family protein-substrate receptor on the vacuolar membrane
               to facilitate the substrate translocation into vacuoles. As the
               newly discovered degradation pathway is dependent upon cytosolic
               Hsp70 (as in CMA) as well as the ESCRT complex, and involves
               invagination of the vacuolar membrane, we refer to it as
               chaperone-mediated microautophagy. Taken together, this study
               has led to the identification of a novel cellular pathway in S.
               cerevisiae that facilitates the clearance of cellular proteins
               under chronic stress.Abbreviations: CMA: chaperone-mediated
               autophagy; CMA-tag: KFERQ motif; ESCRT: endosomal sorting
               complexes required for transport; GAPDH:
               glyceraldehyde-3-phosphate dehydrogenase; GFP: green fluorescent
               protein; HSPA8: heat shock protein 8; LAMP2A:
               lysosomal-associated membrane protein type 2A; Lact-C2:
               lactadherin C2 domain; PAmCherry: photoactivatable mCherry; PBS:
               phosphate-buffered saline; PS: phosphatidylserine; PtdIns4P:
               phosphatidylinositol-4-phosphate; RFP: red fluorescent protein;
               TBST: Tris-buffered saline with Tween 20.",
  journal   = "Autophagy",
  publisher = "Informa UK Limited",
  volume    =  22,
  number    =  7,
  pages     = "1518--1539",
  month     =  jul,
  year      =  2026,
  keywords  = "CMA; Chaperones; KFERQ motifs; microautophagy;
               phosphatidylserine; protein degradation",
  language  = "en"
}