Segregational and structural instability of recombinant plasmid carrying genes for naphthalene degrading pathway.

Samanta, S K and Rani, M and Jain, R K (1998) Segregational and structural instability of recombinant plasmid carrying genes for naphthalene degrading pathway. Letters in applied microbiology, 26 (4). pp. 265-9. ISSN 0266-8254

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Abstract

The stability of recombinant plasmid carrying genes for naphthalene mineralization was determined. A strain of Pseudomonas putida capable of mineralizing naphthalene (Nap+) via salicylate (Sal+) was isolated, and all regulatory and structural genes for the whole pathway were found to be encoded on a 25 kb EcoRI fragment of an approximately 83 kb plasmid present in this strain. The 25 kb EcoRI fragment was cloned into a tetracycline-resistant (TcR) cloning vector pLAFR3 and the recombinant plasmid, pRKJ3 (Nap+, Sal+, TcR), thus obtained was transferred into the plasmid-free strain Pseudomonas putida KT2442 in order to test the stability of the plasmid. Plasmid pRKJ3 was found to be segregationally and/or structurally unstable, depending on the growth conditions. Two types of novel derivative strains having the phenotypes Nap-, Sal+, TcR and Nap-, Sal-, TcR with specific deletions of approximately 2 kb and 18 kb, respectively, were obtained.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Wiley
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	02 Jan 2012 17:26
Last Modified:	02 Jan 2012 17:26
URI:	http://crdd.osdd.net/open/id/eprint/353

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