Genetic transformation of Vitreoscilla sp.

Navani, Naveen K and Joshi, M and Dikshit, Kanak L (1996) Genetic transformation of Vitreoscilla sp. Gene, 177 (1-2). pp. 265-6. ISSN 0378-1119

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Of all the methods customarily used to transform E. coli we found only electroporation to be effective for transformation of the Gram-negative bacterium Vitreoscilla, yielding 5.10(5) transformants/microgram of plasmid DNA. The conditions used were close to those described for E. coli E. coli plasmids are stably maintained in Vitreoscilla. This is the first report of exogenous DNA transfer in Vitreoscilla which opens the way for the application of recombinant-DNA techniques to study this unique group of organisms.

Item Type: Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords: Vitreoscilla stercoraria; Plasmid; Electrotransformation
Subjects: Q Science > QH Natural history > QH426 Genetics
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 16 Dec 2011 08:56
Last Modified: 09 Jan 2015 09:17

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