Micromixing and the steady-state performance of bioreactors using recombinant bacteria--analysis through a reversed two-environment model.

Patnaik, P R (1994) Micromixing and the steady-state performance of bioreactors using recombinant bacteria--analysis through a reversed two-environment model. Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986), 61 (4). pp. 337-42. ISSN 0268-2575

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Abstract

A reversed two-environment model has been used to study micromixing in continuous fermentation for growth and product formation by recombinant bacteria. As an example, an Escherichia coli M72 strain harbouring the plasmid pPLc23trpA1 and producing tryptophan synthetase is considered. With excess substrate, 10% plasmid-free bacteria in the initial broth do not affect the steady-state concentrations of plasmid-containing bacteria but their mass fractions decrease significantly. With a smaller substrate concentration, the mass fractions decrease sharply as the dilution rate increases if micromixing is good; but concentrations still remain high. There is also a clear demarcation between regions of good and poor micromixing. These results are explained and an optimal combination of micromixing and dilution rate is suggested to maximise productivity.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Wiley
Uncontrolled Keywords:	micromixing;continuous fermentation;recombinant bacteria;plasmid stability
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	13 Dec 2011 16:51
Last Modified:	28 Mar 2014 10:53
URI:	http://crdd.osdd.net/open/id/eprint/403

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