Novel assay for screening rifamycin B-producing mutants.

Vohra, R M (1992) Novel assay for screening rifamycin B-producing mutants. Applied and environmental microbiology, 58 (1). pp. 403-4. ISSN 0099-2240

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Abstract

A simple method that allows easy identification of rifamycin B-producing strains is described. This method involves the use of an enzyme, rifamycin oxidase, which converts inactive rifamycin B to active rifamycin S. In this method, colonies to be tested are grown in pairs. The two colonies are then transferred to two plates seeded with a sensitive strain of Staphylococcus aureus, one plate of which contains the enzyme rifamycin oxidase. All paired colonies which show a larger inhibition zone diameter on the enzyme-containing plate are identified as rifamycin B producers.

Item Type:	Article
Additional Information:	Copyright of this article belongs to ASM
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	12 Dec 2011 15:38
Last Modified:	12 Dec 2011 15:38
URI:	http://crdd.osdd.net/open/id/eprint/426

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