Sharma, Amit and Radha Kishan, K V (2011) Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules. FEBS letters, 585 (21). pp. 3465-70. ISSN 1873-3468
PDF
radha11.pdf - Published Version Restricted to Registered users only Download (759Kb) | Request a copy |
Abstract
Protease inhibitors have been extensively used in research to prevent unwanted degradation of proteins during purification and analysis. Here, we report a remarkable discovery of protease inhibitor mediated reformation of peptide bonds by the serine protease inhibitor, PMSF in a diverse set of proteolyzed molecules. Interestingly, the religation reaction in the presence of PMSF occurs in a very short time period and with very high yields of the religated product. We also investigate the plausible mechanism of such a reaction and demonstrate through biochemical studies and X-ray crystallography that proximity of reacting termini is essential for the feasibility of this reaction.
Item Type: | Article |
---|---|
Additional Information: | C 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
Subjects: | Q Science > QR Microbiology |
Depositing User: | Dr. K.P.S.Sengar |
Date Deposited: | 09 Dec 2011 14:12 |
Last Modified: | 09 Dec 2011 14:12 |
URI: | http://crdd.osdd.net/open/id/eprint/445 |
Actions (login required)
View Item |