Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules.

Sharma, Amit and Radha Kishan, K V (2011) Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules. FEBS letters, 585 (21). pp. 3465-70. ISSN 1873-3468

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Abstract

Protease inhibitors have been extensively used in research to prevent unwanted degradation of proteins during purification and analysis. Here, we report a remarkable discovery of protease inhibitor mediated reformation of peptide bonds by the serine protease inhibitor, PMSF in a diverse set of proteolyzed molecules. Interestingly, the religation reaction in the presence of PMSF occurs in a very short time period and with very high yields of the religated product. We also investigate the plausible mechanism of such a reaction and demonstrate through biochemical studies and X-ray crystallography that proximity of reacting termini is essential for the feasibility of this reaction.

Item Type: Article
Additional Information: C 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 09 Dec 2011 14:12
Last Modified: 09 Dec 2011 14:12
URI: http://crdd.osdd.net/open/id/eprint/445

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