Pathway for degradation of 2-chloro-4-nitrophenol in Arthrobacter sp. SJCon.

Arora, Pankaj Kumar and Jain, R K (2011) Pathway for degradation of 2-chloro-4-nitrophenol in Arthrobacter sp. SJCon. Current microbiology, 63 (6). pp. 568-73. ISSN 1432-0991

[img] PDF
arora11.1.pdf - Published Version
Restricted to Registered users only

Download (1761Kb) | Request a copy
Official URL: http://www.springerlink.com/content/023k277u687861...

Abstract

Degradation of 2-Chloro-4-nitrophenol (2C4NP) was studied by Arthrobacter sp. SJCon, isolated from the soil of a pesticide contaminated site. This strain utilized 2C4NP as sole source of carbon and energy and degraded 2C4NP with stoichiometric release of nitrite and chloride ions. A metabolite was detected during the study of 2C4NP degradation and identified as chlorohydroquinone (CHQ) by thin layer chromatography (TLC), high performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC-MS). Inhibition study using 2,2'-dipyridyl showed that CHQ is a terminal aromatic compound in degradation pathway of 2C4NP. CHQ dioxygenase activity was observed in the crude extract of 2C4NP induced cells of the strain SJCon that suggested the cleavage of the CHQ to maleylacetate (MA). Our study clearly showed that Arthrobacter sp. SJCon degraded 2C4NP via formation of CHQ that further cleaved to MA by CHQ dioxygenase. This mechanism of degradation of 2C4NP differs from previously reported degradation pathways of 2C4NP.

Item Type: Article
Additional Information: Copyright of this article belongs to Springer Science.
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 09 Dec 2011 14:12
Last Modified: 03 Apr 2012 06:47
URI: http://crdd.osdd.net/open/id/eprint/447

Actions (login required)

View Item View Item