Promiscuous peptide of 16 kDa antigen linked to Pam2Cys protects against Mycobacterium tuberculosis by evoking enduring memory T-cell response.

Gowthaman, Uthaman and Singh, Vijender and Zeng, Weiguang and Jain, Shweta and Siddiqui, Kaneez F and Chodisetti, Sathi Babu and Gurram, Rama Krishna and Parihar, Pankaj and Gupta, Pushpa and Gupta, Umesh D and Jackson, David C and Agrewala, J N (2011) Promiscuous peptide of 16 kDa antigen linked to Pam2Cys protects against Mycobacterium tuberculosis by evoking enduring memory T-cell response. The Journal of infectious diseases, 204 (9). pp. 1328-38. ISSN 1537-6613

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Abstract

One of the main reasons considered for BCG failure in tuberculosis-endemic areas is impediment by environmental mycobacteria in its processing and generation of memory T-cell response. To overcome this problem, we developed a unique lipopeptide (L91) by linking the promiscuous peptide (sequence 91-110) of 16 kDa antigen of Mycobacterium tuberculosis to Pam2Cys. L91 does not require extensive antigen processing and generates enduring Th1 memory response. This is evidenced by the fact that L91 significantly improved the activation, proliferation, and generation of protective T cells. Furthermore, L91 surmounts the barrier of major histocompatibility complex polymorphism and induces better protection than BCG. This peptide has self-adjuvanting properties and activates dendritic cells. Importantly, L91 activates T cells isolated from purified protein derivative-positive healthy volunteers that responded weakly to free peptide (F91). In essence, L91 can be a potent future vaccine candidate against tuberculosis.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Oxford University press
Subjects:	R Medicine > R Medicine (General)
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	09 Dec 2011 14:13
Last Modified:	19 Mar 2012 07:30
URI:	http://crdd.osdd.net/open/id/eprint/448

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