A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron.

Sharma, Priyanka and Sablok, Kavita and Bhalla, Vijayender and Suri, C Raman (2011) A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron. Biosensors & bioelectronics, 26 (10). pp. 4209-12. ISSN 1873-4235

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Abstract

A disposable electrochemical immunosensor has been developed for the determination of phenyl urea herbicide-diuron using a low cost laser ablated gold electrodes (LC-LAGE) fabricated on polystyrene substrate. The electrodes were electrochemically deposited with prussian blue-gold nanoparticle (PB-GNP) film, and a competitive inhibition immunoassay was performed on LC-LAGE by using a specific hapten-protein conjugate. The binding of available diuron specific antibody on conjugate coated electrode was detected using alkaline phosphatase rabbit anti-IgG antibody. The addition of 1-naphthyl phosphate substrate resulted in the production of electrochemically active product, 1-naphthol, which was monitored using square wave voltammetry technique. The assay exhibited an excellent sensitivity and specificity showing the dynamic response range between 1 ppt and 10 ppm for diuron with detection limit around 1 ppt. This study provides insight into development of a rapid and high-throughput screening of pesticides in environmental samples at a very low cost.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science
Subjects:	Q Science > QD Chemistry
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	09 Dec 2011 06:56
Last Modified:	09 Dec 2011 06:56
URI:	http://crdd.osdd.net/open/id/eprint/470

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