Novel flavohemoglobins of mycobacteria.

Gupta, Sanjay and Pawaria, Sudesh and Lu, Changyuan and Yeh, Syun-Ru and Dikshit, Kanak L (2011) Novel flavohemoglobins of mycobacteria. IUBMB life, 63 (5). pp. 337-45. ISSN 1521-6551

PDF dikshit11.pdf - Published Version Restricted to Registered users only Download (957Kb) | Request a copy

Abstract

Flavohemoglobins (flavoHbs) constitute a distinct class of chimeric hemoglobins in which a globin domain is coupled with a ferredoxin reductase such as FAD- and NADH-binding modules. Structural features and active site of heme and reductase domains are highly conserved in various flavoHbs. A new class of flavoHbs, displaying crucial differences in functionally conserved regions of heme and reductase domains, have been identified in mycobacteria. Mining of microbial genome data indicated that the occurrence of such flavoHbs might be restricted to a small group of microbes unlike conventional flavoHbs that are widespread among prokaryotes and lower eukaryotes. One of the representative flavoHbs of this class, encoded by Rv0385 gene (MtbFHb) of Mycobacterium tuberculosis, has been cloned, expressed, and characterized. The ferric and deoxy spectra of MtbFHb displayed a hexacoordinate state indicating that its distal site may be occupied by an intrinsic amino acid or an external ligand and it may not be involved in nitric oxide detoxification. Phylogenetic analysis revealed that mycobacterial flavoHbs constitute a separate cluster distinct from conventional flavoHbs and may have novel function(s).

Item Type:	Article
Additional Information:	Copyright of this article belongs to Wiley
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	09 Dec 2011 06:56
Last Modified:	09 Dec 2011 06:56
URI:	http://crdd.osdd.net/open/id/eprint/473

Actions (login required)

View Item