Raichand, Revti and Kaur, Ishwinder and Singh, Nitin Kumar and Mayilraj, Shanmugam (2011) Pontibacter rhizosphera sp. nov., isolated from rhizosphere soil of an Indian medicinal plant Nerium indicum. Antonie van Leeuwenhoek, 100 (1). pp. 129-35. ISSN 1572-9699

PDF mayilraj11.5.pdf - Published Version Restricted to Registered users only Download (216Kb) | Request a copy

Abstract

A gram-negative, motile, straight to curved rod shaped, pink pigmented bacterium was isolated from a soil sample collected from the rhizosphere of an Indian medicinal plant, Nerium indicum (Chuvanna arali) and subjected to a detailed polyphasic taxonomic study. The strain, designated as IMTB-1969(T), matched with most of the phenotypic and chemotaxonomic properties of the genus Pontibacter and represents a novel species. The major fatty acids of the strain were monounsaturated iso/anteiso branched C17 fatty acids (45.1%) and iso-C15:0 (16.5%). MK-7 was the predominant isoprenoid quinone. According to 16S rRNA gene sequence analysis, strain IMTB-1969(T) was indicated to belonged to the phylum Bacteroidetes and further phylogenetic analysis revealed that the strain IMTB-1969(T) belongs to the family Cytophagaceae and genus Pontibacter. The highest 16S rRNA gene sequence similarity was with Pontibacter korlensis CCTCC AB 206081(T) (97.2%) and lower sequence similarity was observed with other species in the genus Pontibacter (95.9-94.0%). DNA-DNA relatedness study of the strain IMTB-1969(T) confirmed that it represents a novel species. The G+C content of the genomic DNA was 52.2 (±0.5) mol%. The results of physiological and biochemical tests allowed the genotypic and phenotypic distinction of strain IMTB-1969(T) from its closest phylogenetic relatives. The strain IMTB-1969(T) should be classified as novel species of the genus Pontibacter, for which the name Pontibacter rhizosphera sp. nov. is proposed. The type strain is IMTB-1969(T) (=MTCC 10673(T) = DSM 24399(T)).

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer Science
Uncontrolled Keywords:	Fatty acid methyl ester analysis; DNA–DNA hybridization; Polyphasic taxonomy
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	09 Dec 2011 06:57
Last Modified:	09 Dec 2011 06:57
URI:	http://crdd.osdd.net/open/id/eprint/477

Actions (login required)

View Item