Ranjan, Rajiv Kumar and Rajagopal, Kammara (2010) Efficient ligation and cloning of DNA fragments with 2-bp overhangs. Analytical biochemistry, 402 (1). pp. 91-2. ISSN 1096-0309
Full text not available from this repository. (Request a copy)Abstract
Various methods of ligation are currently available and routinely used by molecular biologists, such as blunt end ligation, cohesive end (two and four overhangs), and ligation of Taq polymerase-derived products. However, there is no efficient method for the cloning of DNA fragments with 2-bp overhangs. We present a simple method for the efficient ligation of DNA fragments with 2-bp overhanging ends, ranging in size from 0.7 to 2.5 kbp. Our method involves the initial heating and flash freezing of the vector-insert DNA mix, and a subsequent unique ligation reaction. This method provides a new molecular biology tool for researchers.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier Science |
| Subjects: | Q Science > QD Chemistry |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 08 Dec 2011 19:30 |
| Last Modified: | 08 Dec 2011 19:30 |
| URI: | http://crdd.osdd.net/open/id/eprint/526 |
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