Expression, purification, crystallization and preliminary X-ray analysis of maleylacetate reductase from Burkholderia sp. strain SJ98.

Chauhan, Archana and Islam, Zeyaul and Jain, R K and Karthikeyan, Subramanian (2009) Expression, purification, crystallization and preliminary X-ray analysis of maleylacetate reductase from Burkholderia sp. strain SJ98. Acta crystallographica. Section F, Structural biology and crystallization communications, 65 (Pt 12). pp. 1313-6. ISSN 1744-3091

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Abstract

Maleylacetate reductase (EC 1.3.1.32) is an important enzyme that is involved in the degradation pathway of aromatic compounds and catalyzes the reduction of maleylacetate to 3-oxoadipate. The gene pnpD encoding maleylacetate reductase in Burkholderia sp. strain SJ98 was cloned, expressed in Escherichia coli and purified by affinity chromatography. The enzyme was crystallized in both native and SeMet-derivative forms by the sitting-drop vapour-diffusion method using PEG 3350 as a precipitant at 293 K. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a = 72.91, b = 85.94, c = 53.07 A. X-ray diffraction data for the native and SeMet-derivative crystal were collected to 2.7 and 2.9 A resolution, respectively.

Item Type: Article
Additional Information: Copyright of this article belongs to Wiley
Subjects: Q Science > QD Chemistry
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 08 Dec 2011 19:32
Last Modified: 03 Apr 2012 06:51
URI: http://crdd.osdd.net/open/id/eprint/538

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