RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe.

Ahmed, Shakil and Singh, Jagmohan (2008) RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe. BioTechniques, 44 (1). 54, 56, 58. ISSN 0736-6205

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Abstract

Here we describe a method that allows selective detection of silent copy transcripts in homothallic strains of Schizosaccharomyces pombe in the presence of the active cassettes. The method involving RT-PCR (reverse transcriptase polymerase chain reaction) exploits our observation that the silent copy transcripts extend beyond the regions of homology to the flanking sequences specific for the donor cassettes, thus allowing design of oligos that are specific for the different donors. The results are validated using a known silencing mutant swi6.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Biotechniques.com
Subjects:	Q Science > QH Natural history > QH301 Biology QH301 Biology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	30 Nov 2011 09:25
Last Modified:	30 Nov 2011 09:26
URI:	http://crdd.osdd.net/open/id/eprint/616

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