Immobilised cellulolytic and hemicellulolytic enzymes from Macrophomina phaseolina

Roy, P. K. and Roy, U. and Dube, Dipak K. (1984) Immobilised cellulolytic and hemicellulolytic enzymes from Macrophomina phaseolina. Journal of Chemical Technology and Biotechnology. Biotechnology, 34 (3). pp. 165-170. ISSN 02643421

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Abstract

Cellulolytic enzymes from Macrophomina phaseolina were immobilised in acrylamide polymer. The immobilised enzyme preparation showed activity towards filter paper and cotton. However, the degree of hydrolysis of highly organised cellulose, particularly cotton, appears to be low in comparison with that of soluble substrate. The kinetic studies of immobilised enzymes indicated the presence of diffusional limitations by the increase in Vmax as the particle size decreased. The operational studies suggested that the immobilised enzymes retained the original activities up to 25–29 times in the reuse cycle

Item Type:	Article
Additional Information:	Copyright of this article belongs to Wiley.
Uncontrolled Keywords:	Macrophomina phaseolina;immobilised Cellulolytic enzymes;operational stability;enzyme reusability
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	11 Jan 2012 14:32
Last Modified:	21 Jun 2013 09:44
URI:	http://crdd.osdd.net/open/id/eprint/656

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