Production of β-glucosidase (cellobiase) by Curvularia sp.

Banerjee, U C (1990) Production of β-glucosidase (cellobiase) by Curvularia sp. Letters in Applied Microbiology, 10 (5). pp. 197-199. ISSN 0266-8254

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Official URL: http://dx.doi.org/10.1111/j.1472-765X.1990.tb01332...

Abstract

A Curvularia sp. isolated from soil was found to produce extracellular β-glucosidase activity when grown in yeast extract, peptone, carboxymethylcellulose (YPC) medium. An initial medium pH of 6·5 and cultivation temperature of 30°C were found to be most suitable for high enzyme productivity. The pH and temperature optima for the enzyme were 4·0 and 70°C, respectively. Under these conditions, the enzyme exhibited a Km (0-nitrophenyl-β-d-glucoside) value of 0.20 mmol/l. Several divalent metal ions inhibited enzyme activity at high concentration. EDTA. also inhibited β-glucosidase activity.

Item Type: Article
Additional Information: Copyright of this article belongs to Wiley.
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 17 Jan 2012 14:29
Last Modified: 27 Mar 2014 10:38
URI: http://crdd.osdd.net/open/id/eprint/657

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