Banerjee, U C (1993) Transformation of rifamycin B with soluble rifamycin oxidase from Curvularia lunata. Journal of Biotechnology, 29 (1-2). pp. 137-143. ISSN 01681656
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Transformation of rifamycin B to rifamycin S using soluble rifamycin oxidase from Curvularia lunata was carried out in shake flask and in a reactor. Optimum transformation temperature was found to be at 50°C but, from the practical point of view, all the transformation reactions were carried out at 28°C. In non-stirring condition it took 50 h to transform the 1% (w/v) solution of rifamycin B, while in stirring condition (200 rpm) it took only 3 h. Five percent enzyme solution (activity 11 IU ml−1) was found to be optimum for the transformation reaction. An aeration rate of 1–1.5 vvm was maintained during transformation reaction in a batch reactor. With higher substrate concentration (3%, w/v) it took more time (7 h) to complete the transformation than with 1% (w/v) rifamycin B (3 h). A few drops of antifoam (polypropylene glycol) was effective in suppressing the foam formed during aeration in the reactor and it did not interfere in the isolation of rifamycin S.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier Science. |
| Uncontrolled Keywords: | Curvularia lunata; Rifamycin B; Soluble rifamycin oxidase; Optimization |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 16 Jan 2012 15:21 |
| Last Modified: | 28 Mar 2012 10:01 |
| URI: | http://crdd.osdd.net/open/id/eprint/664 |
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