Transformation of rifamycin B with soluble rifamycin oxidase from Curvularia lunata

Banerjee, U C (1993) Transformation of rifamycin B with soluble rifamycin oxidase from Curvularia lunata. Journal of Biotechnology, 29 (1-2). pp. 137-143. ISSN 01681656

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Transformation of rifamycin B to rifamycin S using soluble rifamycin oxidase from Curvularia lunata was carried out in shake flask and in a reactor. Optimum transformation temperature was found to be at 50°C but, from the practical point of view, all the transformation reactions were carried out at 28°C. In non-stirring condition it took 50 h to transform the 1% (w/v) solution of rifamycin B, while in stirring condition (200 rpm) it took only 3 h. Five percent enzyme solution (activity 11 IU ml−1) was found to be optimum for the transformation reaction. An aeration rate of 1–1.5 vvm was maintained during transformation reaction in a batch reactor. With higher substrate concentration (3%, w/v) it took more time (7 h) to complete the transformation than with 1% (w/v) rifamycin B (3 h). A few drops of antifoam (polypropylene glycol) was effective in suppressing the foam formed during aeration in the reactor and it did not interfere in the isolation of rifamycin S.

Item Type: Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords: Curvularia lunata; Rifamycin B; Soluble rifamycin oxidase; Optimization
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 16 Jan 2012 15:21
Last Modified: 28 Mar 2012 10:01

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