Effect of glucose and carboxymethylcellulose on growth and rifamycin oxidase production byCurvularia lunata

Banerjee, U C (1993) Effect of glucose and carboxymethylcellulose on growth and rifamycin oxidase production byCurvularia lunata. Current Microbiology, 26 (5). pp. 261-265. ISSN 0343-8651

PDF Banerjee1993.1.pdf - Published Version Restricted to Registered users only Download (293Kb) | Request a copy

Abstract

Curvularia lunata var.aeria was grown in YPD (yeast extract, peptone, and dextrose) medium (pH 6.5) at 28°C with varying concentrations (10–40 g/L) of glucose for the production of rifamycin oxidase. Enzyme activity and glucose concentration were found to be indirectly related to the production of black intracellular pigment by the organism. Depletion of glucose level and rise of culture pH initiate the synthesis of pigment. Carboxymethylcellulose (CMC) was used as a carbon source to improve the enzyme yield, but utilization of the substrate in the reactor was much less. Compared with 10 g/L of CMC in the medium, low or high concentrations of CMC did not yield any better result. Addition of glucose in YPC (yeast extract, peptone, and CMC) medium did not increase the enzyme activity, and glucose was rapidly utilized byC. lunata, forming pellets rather than mycelia. Fulltext Preview

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer Science.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	16 Jan 2012 15:23
Last Modified:	27 Mar 2014 10:34
URI:	http://crdd.osdd.net/open/id/eprint/672

Actions (login required)

View Item