PATNAIK, P.R. (1995) Sensitivity Of Recombinant Fermentation With Run-Away Plasmids: A Structured Analysis Of The Effect Of Dilution Rate. Chemical Engineering Communications, 131 (1). pp. 125-140. ISSN 0098-6445
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Abstract
A recently validated four-compartment model is used to describe intra-cellular dynamics during fermentations with recombinant microorganisms containing temperature-sensitive (run-away) plasmids. The time-dependent sensitivities with respect to the dilution rate have been computed for normal and run-away replication. During normal replication the cells are most sensitive in the early stage of fermentation, and the intra-cellular compartment containing RNA and ribosomes is more sensitive than the other three lumped components. This is explained through a buffering effect. During run-away replication the sensitives are either constant or increase slowly with time, and there is a shift in the relative sensitivities of the intra-cellular components. All sensitivities except that of the substrate increase as the dilution rate increases.
Item Type: | Article |
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Additional Information: | Copyright of this article belongs to Taylor and francis group. |
Uncontrolled Keywords: | Fementation, Plasmids, Dilution rate, Recombinant. |
Subjects: | Q Science > QD Chemistry |
Depositing User: | Dr. K.P.S.Sengar |
Date Deposited: | 27 Jan 2012 15:34 |
Last Modified: | 31 Mar 2014 05:33 |
URI: | http://crdd.osdd.net/open/id/eprint/699 |
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