Sensitivity Of Recombinant Fermentation With Run-Away Plasmids: A Structured Analysis Of The Effect Of Dilution Rate.

PATNAIK, P.R. (1995) Sensitivity Of Recombinant Fermentation With Run-Away Plasmids: A Structured Analysis Of The Effect Of Dilution Rate. Chemical Engineering Communications, 131 (1). pp. 125-140. ISSN 0098-6445

PDF pat95(s).pdf - Published Version Restricted to Registered users only Download (2595Kb) | Request a copy

Abstract

A recently validated four-compartment model is used to describe intra-cellular dynamics during fermentations with recombinant microorganisms containing temperature-sensitive (run-away) plasmids. The time-dependent sensitivities with respect to the dilution rate have been computed for normal and run-away replication. During normal replication the cells are most sensitive in the early stage of fermentation, and the intra-cellular compartment containing RNA and ribosomes is more sensitive than the other three lumped components. This is explained through a buffering effect. During run-away replication the sensitives are either constant or increase slowly with time, and there is a shift in the relative sensitivities of the intra-cellular components. All sensitivities except that of the substrate increase as the dilution rate increases.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Taylor and francis group.
Uncontrolled Keywords:	Fementation, Plasmids, Dilution rate, Recombinant.
Subjects:	Q Science > QD Chemistry
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	27 Jan 2012 15:34
Last Modified:	31 Mar 2014 05:33
URI:	http://crdd.osdd.net/open/id/eprint/699

Actions (login required)

View Item