Strain improvement for the production of a thermostable α-amylase

Sidhu, G.S. and Sharma, P and Chakrabarti, T and Gupta, J.K. (1997) Strain improvement for the production of a thermostable α-amylase. Enzyme and Microbial Technology, 21 (7). pp. 525-530. ISSN 01410229

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Abstract

Bacillus sp. MK716, a thermophilic and amylolytic soil isolate, produced a thermostable α-amylase active even at 100°C. The enzyme yield was increased 40 times through mutagenesis with ethylmethane sulfonate. The mutated gene was cloned in Escherichia coli-pBR322 system, and the 4.8 kb cloned fragment was mapped with restriction enzymes. Subcloning in Bacillus subtilis of a 2.0 kb portion of the cloned fragment resulted in 107 times increased enzyme yield. The recombinant plasmid was completely stable in B. subtilis.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords:	Thermoamylase; mutagenesis; cloning; subcloning; plasmid stability
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	27 Jan 2012 14:57
Last Modified:	19 Jan 2015 05:32
URI:	http://crdd.osdd.net/open/id/eprint/772

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