Production of biosurfactant at mesophilic and thermophilic conditions by a strain of Bacillus subtilis

Makkar, R S and Cameotra, Swaranjit Singh (1998) Production of biosurfactant at mesophilic and thermophilic conditions by a strain of Bacillus subtilis. Journal of Industrial Microbiology and Biotechnology, 20 (1). pp. 48-52. ISSN 1367-5435

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Abstract

The ability of a Bacillus subtilis strain to grow and produce biosurfactant on different carbon and nitrogen sources under thermophilic conditions (45°C) was studied. The strain was able to reduce surface tension to 34 dynes cm-1 on 2% sucrose, and 32 dynes cm-1 on starch after 96 h of growth. The biosurfactant was stable at 100°C and within a wide pH range (3.0–11.0). Biosurfactant formation at mesophilic conditions (30°C) was also studied. The organism was able to produce the maximum amount of biosurfactant when nitrate ions were supplied as the nitrogen source. The potential application of the biosurfactant in oil recovery from desert oil fields, acidic and alkaline environments is demonstrated. The biosurfactant was identical to surfactin as confirmed by TLC and IR analysis.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer Science.
Uncontrolled Keywords:	biosurfactant; thermophilic; Bacillus subtilis
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	30 Jan 2012 17:57
Last Modified:	29 Mar 2012 06:28
URI:	http://crdd.osdd.net/open/id/eprint/785

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