Dispersion-induced behavior in sub-critical operation of a recombinant fed-batch fermentation with run-away plasmids

Patnaik, P R (1998) Dispersion-induced behavior in sub-critical operation of a recombinant fed-batch fermentation with run-away plasmids. Bioprocess Engineering, 18 (3). p. 219. ISSN 0178515X

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Abstract

Fermentations with recombinant bacteria containing run-away plasmids are typically operated alternately above and below a critical temperature. To minimize the risks of run away reactions, it is preferable to keep the high temperature periods as short as possible. In this study the possibility of sustained low temperature (sub-critical) operation in a suitably non-homogeneous broth is analyzed. Fluid dispersion is used as a measure of non-homogeneity. The fed-batch production of g-galactosidase by Escherichia coli containing the plasmid pOU140 and operated below 37 :C is analysed as a model system. To characterize non-homogeneity, an earlier model visualizing the broth as a set of two reactors with internal recycle has been modified for fed-batch fermentation. Three dilution rates, two internal and one external, quantify fluid dispersion. While plasmid replication and fermentation become quenched in sub-critical operation in a well-mixed reactor, with finite dispersion there may be an increase in the concentration of plasmid-containing cells and the recombinant protein. The concentration profiles many also have one or more peaks in the time domain. Thus, sustained fermentation with run-away plasmids appears feasible in a bioreactor with controlled non-homogeneity.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer Science.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	30 Jan 2012 17:56
Last Modified:	12 Jan 2015 04:12
URI:	http://crdd.osdd.net/open/id/eprint/788

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