Analysis of the effect of interruptions in substrate inflow on a fed-batch fermentation with recombinant bacteria

Patnaik, P R (1998) Analysis of the effect of interruptions in substrate inflow on a fed-batch fermentation with recombinant bacteria. Biochemical Engineering Journal, 1 (2). pp. 121-129. ISSN 1369703X

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Abstract

Fermentations employing recombinant bacteria are sensitive to inflow disturbances. In this study, a fed-batch fermentation using a recombinant Escherichia coli strain is analyzed for its performance under interruptions in the flow rate and concentration of the substrate. To enable separate control of the two variables, the experimental scheme has two inflow streams—a concentrated substrate and a diluent—which can be mixed in any desired proportion. The mathematical model expresses the dynamic variations of the overall concentrations of the plasmid-containing and plasmid-free cells in the bioreactor, as well as the intra-cellular concentrations of the plasmid DNA and the recombinant protein (β-galactosidase). The intra-cellular and the extra-cellular variables follow different patterns with time. While interruptions in any one inflow stream reduce β-galactosidase production, simultaneous failure and restart of both streams promote its formation. This result may be explained by analogy with previous studies with feed cycling, and suggests that short-duration interruptions may have fortuitously beneficial effects.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords:	Fed-batch fermentation; Recombinant β-galactosidase; Feed interruptions; Dynamic effects
Subjects:	Q Science > QD Chemistry
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	30 Jan 2012 17:56
Last Modified:	09 Jan 2015 10:33
URI:	http://crdd.osdd.net/open/id/eprint/789

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