A sufficiency analysis of asymptotic stability in continuous recombinant fermentation with auxotrophic mutants

Patnaik, P R (1998) A sufficiency analysis of asymptotic stability in continuous recombinant fermentation with auxotrophic mutants. Biochemical Engineering Journal, 2 (2). pp. 157-160. ISSN 1369703X

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Abstract

Fermentations employing genetically modified microorganisms are sensitive to the disturbances likely in large bioreactors. Since these disturbances may affect the competition between recombinant cells and plasmid-free cells, with consequent effects on plasmid stability and reactor stability, it is useful to identify regions of asymptoticstability (RAS) which do not vary with shifts in the operating condition. Using a model for continuousfermentation with auxotrophicmutants, sufficient conditions have been derived for such operationally invariant RAS. Plots for Saccharomyces cerevisiae containing the pBR322 plasmid show that improving the segregational stability makes the fermentation more vulnerable to disturbances. Thus, real reactor operation must balance both kinds of stability.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords:	Continuousrecombinantfermentation; Disturbances; Region of asymptoticstability
Subjects:	Q Science > QD Chemistry
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	30 Jan 2012 17:56
Last Modified:	12 Jan 2015 04:23
URI:	http://crdd.osdd.net/open/id/eprint/790

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