Improved stability and expression of a recombinant shuttle plasmid in Escherichia coli during fedbatch cultivation.

Nayak, D.P. and Vyas, V V (1999) Improved stability and expression of a recombinant shuttle plasmid in Escherichia coli during fedbatch cultivation. World Journal of Microbiology and Biotechnology, 15 (1). pp. 65-71. ISSN 09593993

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Abstract

The effect of higher cell densities on the expression and segregational stability of a recombinant E. coli- B. subtilisshuttle plasmid coding for carboxymethylcellulase (CMCase) activity, was studied in E. coli DH5. Of the various feeding policies adopted for maximal expression and stability, exponential feeding resulted in the highest biomass of 15g dry cell weight (DCW) l–1 and plasmid stability of 45%. A CMCase activity of 11400 Uml–1 was achieved as compared to 230 Uml–1 during batch cultivation. In the case of other feeding strategies viz., constant feeding, linear feeding or intermittent feeding, the plasmid stability varied between 20% to 60%. Biomass achieved ranged from 5.0 g DCW l–1 to 9.0 g DCW l–1 and enzyme activities were between 2550 Uml–1 and 6000 Uml–1.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer Science.
Uncontrolled Keywords:	Escherichia coli, fedbatch cultivation, growth rate, high cell density, plasmid stability
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	31 Jan 2012 08:43
Last Modified:	09 Jan 2015 10:58
URI:	http://crdd.osdd.net/open/id/eprint/810

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