Cloning, expression, and sequence analysis of the gene encoding the alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic Bacillus sp. Strain NG-27.

Gupta, N and Reddy, V S and Maiti, S and Ghosh, A (2000) Cloning, expression, and sequence analysis of the gene encoding the alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic Bacillus sp. Strain NG-27. Applied and environmental microbiology, 66 (6). pp. 2631-5. ISSN 0099-2240

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Abstract

Alkalophilic Bacillus sp. strain NG-27 produces a 42-kDa endoxylanase active at 70 degrees C and at a pH of 8.4. The gene for this endoxylanase was cloned and sequenced. The gene contained one open reading frame of 1,215 bases. An active site characteristic of the family 10 beta-glycanases was recognized between amino acids 303 and 313, with the active glutamate at position 310. Though highly thermostable, the enzyme contains no cysteine residue.

Item Type:	Article
Additional Information:	Copyright of this article belongs to ASM.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	02 Feb 2012 05:03
Last Modified:	02 Feb 2012 05:03
URI:	http://crdd.osdd.net/open/id/eprint/834

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