Plasmid stability analysis in a non-homogeneous bioreactor for a recombinant fed-batch fermentation

Patnaik, P R (1999) Plasmid stability analysis in a non-homogeneous bioreactor for a recombinant fed-batch fermentation. The Canadian Journal of Chemical Engineering, 77. pp. 602-607. ISSN 00084034

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model incorporating segregational loss of the plasmid pPLc23rrpAl and imperfect macromixing of the broth. These features become significant in large fermentation vessels, where fluid circulation in the bioreactor influences the rates of cell growth and productivity. A simple model consisting of two interacting reactors describes the degree of macromixing, which is characterized by the respective (internal) dilution rates. Controlled imperfect macromixing is superior to other methods in arresting, and even reversing, the normal decline in the concentration of recombinant cells, thereby providing a method to exploit the non-homogeneity of the broth in large bioreactors. Some physical implications are discussed. On a analyst la fermentation en alimentation discontinue pour la synthttase de tryptophan par une souche de Escherichia coli recombinante au moyen d’un modkle incorporant la perte stgrkgationnelle du plasmide pPLc23ttpA I et le macromklange imparfait du bouillon. Ces caracttristiques deviennent significatives dans les rtservoirs de fermentation de grande taille, oh la circulation des fluides dans le biorkacteur influence la vitesse de croissance et la productivitt des cellules. Un modble simple composk de deux rtacteurs qui interagissent dtcrit le degrt de macromtlange, qui est caracttrisk par les vitesses de dilution respectives (internes). Le macromklange imparfait contr6lk est suptrieur ?I celui d’autres mtthodes pour stopper, et m&me inverser, le dkclin normal dans la concentration de cellules recombinantes, ce qui fournit une mtthode pour exploiter la non-homogkntitt du bouillon dans les grands biortacteurs. Certaines implications physiques sont analyskes.

Item Type: Article
Additional Information: Copyright of this article belongs to Wiley.
Uncontrolled Keywords: fed-batch fermentation, recombinant E. coli, imperfect macromixing, plasmid stability.
Subjects: Q Science > QD Chemistry
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 02 Feb 2012 16:30
Last Modified: 02 Feb 2012 16:30

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