An investigation on glycerol biosynthesis by an osmophilic yeast in a bioreactor

Sahoo, Debendra K and Agarwal, Gopal P (2001) An investigation on glycerol biosynthesis by an osmophilic yeast in a bioreactor. Process Biochemistry, 36 (8-9). pp. 839-846. ISSN 13595113

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Glycerol production by osmophilic yeasts has a significant potential for large-scale production. The effects of important parameters including initial glucose concentration, carbon/nitrogen (C/N) ratio, aeration rate and pH were investigated in a 6 l bioreactor with the objective of a further scale-up of the process. The kinetics of glycerol biosynthesis were analysed as a function of these parameters. The initial glucose concentration significantly affected both cell growth and glycerol accumulation. A high C/N ratio resulted in poor cell growth, slow glucose uptake and a small amount of glycerol accumulation, whereas a decrease in C/N ratio favoured increased rates of growth, glucose uptake and production of high concentrations of ethanol but not necessarily increased glycerol production. Control of pH was not necessary, but aeration rate played an important role in the yield and productivity of glycerol.

Item Type: Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords: Candida magnoliae I2B; Effect of aeration; Effect of C/N ratio; Glycerol; Osmophilic yeast; Polyols
Subjects: Q Science > QD Chemistry
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 03 Feb 2012 16:14
Last Modified: 03 Feb 2012 16:14

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