Synthesis of enhanced biosurfactant by Bacillus subtilis MTCC 2423 at 45°C by foam fractionation

Makkar, R S and Cameotra, Swaranjit Singh (2001) Synthesis of enhanced biosurfactant by Bacillus subtilis MTCC 2423 at 45°C by foam fractionation. Journal of Surfactants and Detergents, 4 (4). pp. 355-357. ISSN 1097-3958

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Abstract

fermentor was carried out using Bacillus subtilis MTCC 2423 at 45°C. A good yield was obtained from sucrose (2%) substrate fermentation by continuous removal of the product by foam fractionation. The biosurfactant was recovered from collapsed foam by acid precipitation. The biosurfactant yield (4.5 g/L) was about 4.5 times higher than the yield (ca. 1 g/L) obtained by shake-flask fermentation. Surface activity of the collapsed foam was very high, and total surface activity was observed in the collapsed foam. The structural characterization of this biosurfactant produced at 45°C by the strain used in this study was recently reported. The biosurfactant was analyzed by thin-layer chromatography, infrared, 1H nuclear magnetic resonance, and mass spectroscopy and was found to be identical to surfactin, a lipopeptide surfactant.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer Science.
Uncontrolled Keywords:	Bacillus, enhanced biosurfactant, foam fractionation, surfactin, thermophilic.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	03 Feb 2012 16:13
Last Modified:	29 Mar 2012 06:37
URI:	http://crdd.osdd.net/open/id/eprint/875

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