Use of Liposomes as an Immunopotentiating Delivery System: in Perspective of Vaccine Development

Owais, M and Masood, A. K. and Agrewala, J N and Bisht, D and Gupta, C M (2001) Use of Liposomes as an Immunopotentiating Delivery System: in Perspective of Vaccine Development. Scandinavian Journal of Immunology, 54 (1-2). pp. 125-132. ISSN 0300-9475

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Official URL: http://dx.doi.org/10.1046/j.1365-3083.2001.00944.x

Abstract

Liposomes have been widely used to deliver antigens to the antigen-presenting cells (APCs) and also to modify their immunological behaviour in model animals. We recently demonstrated the potential of yeast lipid liposomes to undergo membrane┬▒membrane fusion with cytoplasmic membrane of the target cells. Interestingly, studies in the present report revealed that antigen encapsulated in yeast lipid liposomes could be successfully delivered simultaneously into the cytosolic as well as endosomal processing pathways of APCs, leading to the generation of both CD41 T helper and CD81 cytotoxic T cells. In contrast, encapsulation of same antigen in egg phosphatidyl-choline (PC) liposomes, just like its free form, has inefficient access to the cytosolic pathway of major histocompatibility complex (MHC) I dependent antigen presentation and failed to generate antigen specific CD81 cytotoxic T-cell response. However, both egg PC as well as yeast lipid liposomes have elicited strong antigen specific antibody responses in immunized animals. These results imply usage of liposome encapsulated antigen as potential candidate vaccine capable of eliciting both cell mediated as well as humoral immune responses.

Item Type: Article
Additional Information: Copyright of this article belongs to Wiley.
Subjects: Q Science > QR Microbiology > QR180 Immunology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 03 Feb 2012 16:12
Last Modified: 09 Jan 2015 09:21
URI: http://crdd.osdd.net/open/id/eprint/877

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