Condition number analysis for a preliminary evaluation of recombinant fermentation performance

Patnaik, P R (2002) Condition number analysis for a preliminary evaluation of recombinant fermentation performance. Biotechnology Letters, 24 (2). pp. 115-119. ISSN 01415492

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Abstract

Fermentations utilizing genetically modified microbes require accurate monitoring and control. This is done through a control algorithm based on a process model; control usually involves continual measurements and manipulations of the substrate feed rate and its concentration. Since variations in the data, due to the limitations on measurements or external influences, affect the performance, it is useful to have a quick evaluation of these effects before sensitivities or control strategies are studied in detail. Condition numbers provide a convenient way to do this. They have been employed here for a continuous fermentation for -galactosidase production by Saccharomyces cerevisiae containing the plasmid pSXR125. When all recombinant cells have the same number of copies of the plasmid, a selective medium permits less precision in measurement and manipulation than a non-selective medium, but this is reversed when there are two sub-populations with different numbers of plasmids. The more stringent requirement for a selective medium in the latter case is, however, offset by its greater productivity.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer Science.
Uncontrolled Keywords:	condition number, medium selection, plasmid distribution, recombinant fermentation
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	06 Feb 2012 18:16
Last Modified:	09 Jan 2015 09:59
URI:	http://crdd.osdd.net/open/id/eprint/903

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