Patnaik, P R (2002) Steady state performance and sensitivity of a continuous recombinant fermentation with plasmid copy number multiplicity. Indian Jouranl of Biotechnology, 1. pp. 263-269.
Full text not available from this repository. (Request a copy)Abstract
Fermentations utilizing genetically modified cells usually contain two or more fractions of plasmid-bearing cells differing in the plasmid copy number. Competition among these cells and those without the plasmid may lead to a different performance than in the more 'ideal' situation where all recombinant cells have the same number of plasmids. A recent study considered the steady state sensitivities to the feed concentration of substrate and the dilution rate for a continuous fermentation with Saccharomyces cerevisiae XK1-C2 harbouring the plasmid pSXR125, which synthesizes b-galactosidase, with a uniform copy number. A similar analysis has been applied here to the same fermentation with a binomial distribution in the copy number. Although the steady states and their sensitivities are different from those for a uniform distribution, the dilution rate is still the favoured manipulated variable for control. While a selective medium generates a superior performance, its higher sensitivities require rapid on-line monitoring and anticipatory control.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier Science. |
| Uncontrolled Keywords: | continuous fermentation, plasmid copy number, steady state, sensitivity |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 13 Feb 2012 15:20 |
| Last Modified: | 13 Feb 2012 15:20 |
| URI: | http://crdd.osdd.net/open/id/eprint/908 |
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