Sharma, Rohit and Soni, S K and Vohra, R M and Jolly, R S and Gupta, L.K. and Gupta, J.K. (2002) Production of an extracellular alkaline lipase from a new Bacillus sp. RSJ1 and its application in ester hydrolysis. Indian Journal of Microbiology, 42 (1). pp. 49-54. ISSN 0046-8991
Full text not available from this repository. (Request a copy)Abstract
A thermophilic isolate, Bacillus sp. RSJ1 from hot-spring, grew in temperature range of 40 - 65°C and a pH range of 5 -12 with the optima at 50°C and pH 9. The organism grew logarithmically at 50°C from 2 to 13 h in shakecultures before entering into the stationary phase. The pattern of extracellular alkaline lipase production was found to be growth associated and its secretion started as soon as the organism entered the logarithmic phase with the maximum release (9.2 U mL-1) in the late exponential phase (at 12 h). Production of lipase was substantially enhanced when the type, concentration and sources of carbon, nitrogen and surfactant were consecutively optimised. Cotton-seed oil at a concentration of 0.75% in combination with 0.5% Tween 80 and yeast extract in combination with peptone (0.75% each) were found best sources of carbon and nitrogen giving maximum lipase yield. None of the metal ion salts, other than calcium chloride and sodium chloride, incorporated in the production medium improved the lipase yield. The enzyme was found to catalyze reactions of ester hydrolysis with lighter substituents like the chloro and methyl, but was unable to recognize the bulkier substituents like the bromo and the phenyl. Lipase catalyzed the hydrolysis of the esters - butyl phenylacetate, butyl 2-chloropropanoate and butyl 2-methylbutyrate.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Association of Microbiologists of India. |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 13 Feb 2012 15:15 |
| Last Modified: | 13 Feb 2012 15:15 |
| URI: | http://crdd.osdd.net/open/id/eprint/926 |
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