Strategies for direct attachment of hapten to a polystyrene support for applications in enzyme-linked immunosorbent assay (ELISA)

Kaur, J and Singh, K.V. and Raje, Manoj and Varshney, Grish C and Suri, C Raman (2004) Strategies for direct attachment of hapten to a polystyrene support for applications in enzyme-linked immunosorbent assay (ELISA). Analytica Chimica Acta, 506 (2). pp. 133-135. ISSN 00032670

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Abstract

A new method describing direct attachment of carboxylated haptens on a polystyrene support, using 3-aminopropyltriethoxysilane (3-APTES) as a linker, is reported. The hapten coated polystyrene support showed excellent stability as a function of the buffer pH and reaction time, and was successfully used to demonstrate its application in enzyme-linked immunosorbent assay (ELISA).

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords:	Hapten; Polystyrene; Silanization; ELISA
Subjects:	Q Science > QD Chemistry
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	14 Feb 2012 10:30
Last Modified:	14 Feb 2012 10:30
URI:	http://crdd.osdd.net/open/id/eprint/951

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