A novel family of transporters mediating the transport of glutathione derivatives in plants.

Zhang, Ming-Yong and Bourbouloux, Andrée and Cagnac, Olivier and Srikanth, Chittur V and Rentsch, Doris and Bachhawat, Anand K and Delrot, Serge (2004) A novel family of transporters mediating the transport of glutathione derivatives in plants. Plant physiology, 134 (1). pp. 482-91. ISSN 0032-0889

PDF bachhawat2004.pdf - Published Version Restricted to Registered users only Download (253Kb) | Request a copy

Abstract

Uptake and compartmentation of reduced glutathione (GSH), oxidized glutathione (GSSG), and glutathione conjugates are important for many functions including sulfur transport, resistance against biotic and abiotic stresses, and developmental processes. Complementation of a yeast (Saccharomyces cerevisiae) mutant (hgt1) deficient in glutathione transport was used to characterize a glutathione transporter cDNA (OsGT1) from rice (Oryza sativa). The 2.58-kb full-length cDNA (AF393848, gi 27497095), which was obtained by screening of a cDNA library and 5'-rapid amplification of cDNA ends-polymerase chain reaction, contains an open reading frame encoding a 766-amino acid protein. Complementation of the hgt1 yeast mutant strain with the OsGT1 cDNA restored growth on a medium containing GSH as the sole sulfur source. The strain expressing OsGT1 mediated [3H]GSH uptake, and this uptake was significantly competed not only by unlabeled GSSG and GS conjugates but also by some amino acids and peptides, suggesting a wide substrate specificity. OsGT1 may be involved in the retrieval of GSSG, GS conjugates, and nitrogen-containing peptides from the cell wall.

Item Type:	Article
Additional Information:	Copyright of this article belongs to American Society of Plant Biologists.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	14 Feb 2012 10:29
Last Modified:	14 Feb 2012 10:29
URI:	http://crdd.osdd.net/open/id/eprint/957

Actions (login required)

View Item