Emergence and continued circulation of dengue-2 (genotype IV) virus strains in northern India.

Dash, Paban Kumar and Parida, Man Mohan and Saxena, Parag and Kumar, Manoj and Rai, Arvind and Pasha, S.T and Jana, Asha Mukul (2004) Emergence and continued circulation of dengue-2 (genotype IV) virus strains in northern India. Journal of medical virology, 74 (2). pp. 314-22. ISSN 0146-6615

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Abstract

Dengue (DEN) is an acute mosquito borne viral disease of mankind. Off late it has become an important public health concern in Southeast Asia. Although, all the four known dengue virus serotypes (DEN-1 to 4) are reported from time to time, in the recent past, DEN-2 has emerged as the predominant type, being the causative agent of several outbreaks of dengue fever (DF) and dengue haemorrhagic fever (DHF) in India. To elucidate the true molecular epidemiology of these viruses, we have sequenced C-prM gene junction (454 nucleotides) of 11 DEN-2 viruses directly from patient serum. The C-prM gene junction was amplified initially by reverse transcription-polymerase chain reaction followed by automated DNA sequencing. These sequences provide unique information with regard to molecular epidemiology when compared to other DEN-2 sequences from diverse geographic origins. The sequence analysis revealed that most of the mutations in this region remained silent, except a few at the carboxy-terminal of the capsid. Reported phylogenetic analysis classifies DEN-2 viruses into five distinct genotypes. The Gwalior DEN-2 viruses, included in the present study were classified into genotype-IV, and were found to be most closely related to Delhi 1996 DEN-2 viruses and FJ 10/11 strains prevalent in the Fujian state of China. However, two earlier Indian isolates of DEN-2 were classified into genotype-V. The present study indicates that genotype V of DEN-2 has been replaced by genotype IV during the past decade, which continues to circulate silently in north India, and have the potential to reemerge and cause major epidemics of DF and DHF.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Wiley.
Uncontrolled Keywords:	DEN-2; C-prM; sequencing; molecular epidemiology; phylogeny
Subjects:	Q Science > QR Microbiology > QR355 Virology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	14 Feb 2012 12:16
Last Modified:	09 Jan 2015 09:44
URI:	http://crdd.osdd.net/open/id/eprint/965

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