Kumar, Manoj and Pasha, S.T and Mittal, V. and Arya, S.C. and Agrawal, N. and Bhattacharya, D. and Shiv, Lal and Rai, Arvind (2004) Unusual emergence of guate98-like molecular subtype of DEN-3 during 2003 dengue outbreak in Delhi. Dengue Bulletin, 28. pp. 457-461.
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Abstract
With a view to identifying the molecular subtype of the circulating dengue virus responsible for a major outbreak of dengue fever (DF) / dengue haemorrhagic fever (DHF) in and around Delhi during the postmonsoon period in 2003, 32 serum samples were collected from clinically suspected cases. These were subjected to reverse transcription/polymerase chain reaction (RT/PCR) for amplification of 511 bp CPreM gene region of the dengue virus. Seven specimens, yielding a satisfactory quantum of viral RNA, were subsequently processed for automated nucleotide sequencing. Five of the seven analysed isolates showed close DNA sequence homology with Guate96-98 strains of DEN-3 virus, whereas two turned out to be genotype IV of DEN-2. Earlier, DEN-2 (genotype IV) had been identified as the etiological agent during a major DF/DHF outbreak in Delhi in 1996 and also in 2000. Though DEN-2 continues to prevail, DEN-3, having a close sequence homology with Guate96-98 strains, seems to have entered India for the first time in late 2003, resulting in a major DF/DHF outbreak. How the Guate96-98 strain of DEN-3 entered India remains to be linked epidemiologically.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 14 Feb 2012 12:16 |
| Last Modified: | 09 Jan 2015 11:38 |
| URI: | http://crdd.osdd.net/open/id/eprint/966 |
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