Sonawane, Vijay Chintaman (2005) A mini-scale method for assay of D-amino acid oxidase activity inTrigonopsis variabilisagainst cephalosporin C. Biocatalysis and Biotransformation, 23 (2). pp. 117-121. ISSN 1024-2422
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A mini-scale method for the estimation of D-amino acid oxidase activity against cephalosporin C was developed. The method allows implementation of the oxygenation and thorough mixing in a microfuge tube scale reactor. Dilution of reaction products in a buffer containing 0.03% hydrogen peroxide converted the intermediate product of the enzymatic reaction, α-keto adipyl-7-aminocephalosopranic acid (KA-7-ACA) to glutaryl-7-aminocephalosporanic acid (GL-7-ACA). In combination with high performance liquid chromatography, D-amino acid oxidase activity in the whole cells of Trigonopsis variabilis could be measured based on cephalosporin consumed in the reaction or the GL-7-ACA formed. This method could also be used for assay of D-amino acid oxidase activity in crude and soluble preparations
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Taylor and Francis Group. |
| Uncontrolled Keywords: | Assay, biotransformation, cephalosporin C, D-amino acid oxidase; Trigonopsisvariabilis |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 15 Feb 2012 14:36 |
| Last Modified: | 15 Feb 2012 14:36 |
| URI: | http://crdd.osdd.net/open/id/eprint/996 |
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