<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "IFN-gamma-induced expression of MUC4 in pancreatic cancer cells is mediated by STAT-1 upregulation: a novel mechanism for IFN-gamma response."^^ . "MUC4 is a transmembrane mucin, which is aberrantly expressed in pancreatic adenocarcinoma with no detectable expression in the normal pancreas. Here, we present a novel mechanism of IFN-gamma-induced expression of MUC4 in pancreatic cancer cells. Our studies highlight the upregulation of STAT-1 as a basis for MUC4 induction and demonstrate that its activation and upregulation by IFN-gamma are two distinct, albeit temporally integrated, signalling events that drive the selective induction of IRF-1 and MUC4, respectively, within a single cell system. The profile of interferon regulatory factor (IRF)-1 gene induction by IFN-gamma is consistent with its rapid transactivation by phospho-Y701-STAT-1. In contrast, the induction of the MUC4 mucin gene expression is relatively delayed, and occurs only in response to an increase in STAT-1 expression. A progressive binding of STAT-1 to various gamma-interferon-activated sequences (GAS) in the MUC4 promoter is observed in chromatin immunoprecipitation assay, indicating its direct association. Stimulation of STAT-1 expression by double-stranded polynucleotides or ectopic expression is shown to induce MUC4 expression, without Y701 phosphorylation of STAT-1. This effect is abrogated by short interfering RNA (siRNA)-mediated inhibition of STAT-1 expression, supporting further the relevance of STAT-1 in MUC4 regulation. In conclusion, our findings identify a novel mechanism for MUC4 regulation in pancreatic cancer cells and unfold new perspectives on the foundation of IFN-gamma-dependent gene regulation."^^ . "2007-11-08" . . "26" . "51" . . "Nature Publishing Group"^^ . . . "Oncogene"^^ . . . "09509232" . . . . . . . . . . . . . . . . . . . . . . . . . . . . "S K"^^ . "Batra"^^ . "S K Batra"^^ . . "Grish C"^^ . "Varshney"^^ . "Grish C Varshney"^^ . . "P P"^^ . "Mehta"^^ . "P P Mehta"^^ . . "Mahefatiana"^^ . "Andrianifahanana"^^ . "Mahefatiana Andrianifahanana"^^ . . "C"^^ . "Nemos"^^ . "C Nemos"^^ . . "A P"^^ . "Singh"^^ . "A P Singh"^^ . . "M P"^^ . "Ponnusamy"^^ . "M P Ponnusamy"^^ . . "N"^^ . "Moniaux"^^ . "N Moniaux"^^ . . . . . . "IFN-gamma-induced expression of MUC4 in pancreatic cancer cells is mediated by STAT-1 upregulation: a novel mechanism for IFN-gamma response. (PDF)"^^ . . . . . "IFN-gamma-induced expression of MUC4 in pancreatic cancer cells is mediated by STAT-1 upregulation: a novel mechanism for IFN-gamma response. (Indexer Terms)"^^ . . . . . . "IFN-gamma-induced expression of MUC4 in pancreatic cancer cells is mediated by STAT-1 upregulation: a novel mechanism for IFN-gamma response. (Image (PNG))"^^ . . . . . . "IFN-gamma-induced expression of MUC4 in pancreatic cancer cells is mediated by STAT-1 upregulation: a novel mechanism for IFN-gamma response. (Image (PNG))"^^ . . . . . . "IFN-gamma-induced expression of MUC4 in pancreatic cancer cells is mediated by STAT-1 upregulation: a novel mechanism for IFN-gamma response. (Image (PNG))"^^ . . . . . . "IFN-gamma-induced expression of MUC4 in pancreatic cancer cells is mediated by STAT-1 upregulation: a novel mechanism for IFN-gamma response. (Image (PNG))"^^ . . . . . "HTML Summary of #1048 \n\nIFN-gamma-induced expression of MUC4 in pancreatic cancer cells is mediated by STAT-1 upregulation: a novel mechanism for IFN-gamma response.\n\n" . "text/html" . . . "QR Microbiology"@en . .