TY  - JOUR
N1  - Copyright of this article belongs to ASBMB.
ID  - open1059
UR  - http://dx.doi.org/10.1074/jbc.M608328200
IS  - 5
A1  - Raje, Chaaya Iyengar
A1  - Kumar, S.
A1  - Harle, A.
A1  - Nanda, Jagpreet S
A1  - Raje, Manoj
Y1  - 2006///
N2  - The reticuloendothelial system plays a major role in iron metabolism. Despite this, the manner in which macrophages handle iron remains poorly understood. Mammalian cells utilize transferrin-dependent mechanisms to acquire iron via transferrin receptors 1 and 2 (TfR1 and TfR2) by receptor-mediated endocytosis. Here, we show for the first time that the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is localized on human and murine macrophage cell surface. The expression of this surface GAPDH is regulated by the availability of iron in the medium. We further demonstrate that this GAPDH interacts with transferrin and the GAPDH-transferrin complex is subsequently internalized into the early endosomes. Our work sheds new light on the mechanisms involved in regulation of iron, vital for controlling numerous diseases and maintaining normal immune function. Thus, we propose an entirely new avenue for investigation with respect to transferrin uptake and regulation mechanisms in macrophages. 
PB  - ASBMB
JF  - Journal of Biological Chemistry
VL  - 282
SN  - 00219258
TI  - The Macrophage Cell Surface Glyceraldehyde-3-phosphate Dehydrogenase Is a Novel Transferrin Receptor
SP  - 3252
AV  - restricted
EP  - 3261
ER  -