creators_name: Vyas, Rajan
creators_name: Kumar, Vijay
creators_name: Panjikar, Santosh
creators_name: Karthikeyan, Subramanian
creators_name: Kishan, K V Radha
creators_name: Tewari, Rupinder
creators_name: Weiss, Manfred S
type: article
datestamp: 2012-02-21 18:06:20
lastmod: 2012-02-21 18:06:20
metadata_visibility: show
title: Purification, crystallization and preliminary X-ray diffraction analysis of aspartate semialdehyde dehydrogenase (Rv3708c) from Mycobacterium tuberculosis.
ispublished: pub
subjects: QD
full_text_status: restricted
keywords: aspartate semialdehyde dehydrogenase; Mycobacterium tuberculosis; Rv3708c.
note: Copyright of this article belongs toInternational Union of Crystallography.
abstract: Aspartate semialdehyde dehydrogenase from Mycobacterium tuberculosis (Asd, ASADH, Rv3708c), which is the second enzyme in the lysine/homoserine-biosynthetic pathways, has been expressed heterologously in Escherichia coli. The enzyme was purified using affinity and gel-filtration chromatographic techniques and crystallized in two different crystal forms. Preliminary diffraction data analysis suggested the presence of up to four monomers in the asymmetric unit of the orthorhombic crystal form A and of one or two monomers in the cubic crystal form B.
date: 2008-03-01
date_type: published
publication: Acta crystallographica. Section F, Structural biology and crystallization communications
volume: 64
number: Pt 3
publisher: International Union of Crystallography
pagerange: 167-70
refereed: TRUE
issn: 1744-3091
official_url: http://scripts.iucr.org/cgi-bin/paper?S1744309108002753
related_url_url: http://scripts.iucr.org/cgi-bin/paper?S1744309108002753
citation:   Vyas, Rajan and Kumar, Vijay and Panjikar, Santosh and Karthikeyan, Subramanian and Kishan, K V Radha and Tewari, Rupinder and Weiss, Manfred S  (2008) Purification, crystallization and preliminary X-ray diffraction analysis of aspartate semialdehyde dehydrogenase (Rv3708c) from Mycobacterium tuberculosis.  Acta crystallographica. Section F, Structural biology and crystallization communications, 64 (Pt 3).  pp. 167-70.  ISSN 1744-3091     
document_url: http://crdd.osdd.net/open/1077/1/karthikeyan2008.pdf