TY - JOUR N1 - Copyright of this article belongs to SGM. ID - open1104 UR - http://ijs.sgmjournals.org/content/60/4/721.full.pdf+html IS - Pt 4 A1 - Anil Kumar, P A1 - Srinivas, T N R A1 - Madhu, S A1 - Manorama, R A1 - Shivaji, S Y1 - 2010/04// N2 - A novel Gram-stain-negative, rod-shaped, non-motile bacterium, strain LW1(T), was isolated from a water sample collected at a depth of 3.5 m from Lonar Lake, Buldhana district, Maharashtra, India. The cell suspension was reddish-orange due to the presence of carotenoids. Strain LW1(T) was positive for catalase, oxidase, ornithine decarboxylase and lysine decarboxylase and negative for gelatinase, urease and lipase. Fatty acids were dominated by branched-chain fatty acids (>76 %), with a high abundance of iso-C(15 : 0) (48 %), anteiso-C(15 : 0) (7 %) and iso-C(17 : 0) 3-OH (11 %). Strain LW1(T) contained MK-4 and MK-7 as the major respiratory quinones and phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine as the major phospholipids. A blast sequence similarity search based on 16S rRNA gene sequences indicated that members of the genera Belliella and Aquiflexum were the nearest phylogenetic neighbours with similarities of 91.8-92.3 %. Phylogenetic analyses indicated that strain LW1(T) formed a deep-rooted lineage distinct from the clades represented by the genera Belliella, Aquiflexum, Cyclobacterium, Echinicola and Algoriphagus. Based on the above-mentioned phenotypic and phylogenetic characteristics, it is proposed that strain LW1(T) represents a novel species in a new genus, Indibacter alkaliphilus gen. nov., sp. nov. (type strain LW1(T)=KCTC 22604(T)=CCUG 57479(T)). The genomic DNA G+C content of strain LW1(T) is 42.7+/-1 mol%. PB - Society for General Microbiology (SGM) JF - International journal of systematic and evolutionary microbiology VL - 60 SN - 1466-5026 TI - Indibacter alkaliphilus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake. SP - 721 AV - restricted EP - 6 ER -