TY  - JOUR
N1  - Copyright of this article belongs to Wiley.
ID  - open1116
UR  - http://onlinelibrary.wiley.com/doi/10.1111/j.1758-2229.2009.00129.x/abstract
IS  - 1
A1  - Abd, Hadi
A1  - Valeru, Soni Priya
A1  - Sami, Susan Marouf
A1  - Saeed, Amir
A1  - Raychaudhuri, Saumya
A1  - Sandström, Gunnar
Y1  - 2010/02//
N2  - Vibrio mimicus is a Gram-negative bacterium, which causes gastroenteritis and is closely related to Vibrio cholerae. The environmental reservoir of this bacterium is far from defined. Acanthamoeba as well as Vibrio species are found in diverse aquatic environments. The present study was aimed to investigate the ability of A. castellanii to host V. mimicus, the role of bacterial protease on interaction with A. castellanii and to disclose the ability of cysts to protect intracellular V. mimicus. Co-cultivation, viable counts, gentamicin assay, electron microscopy and statistical analysis showed that co-cultivation of wild type and luxO mutant of V. mimicus strains with A. castellanii did not inhibit growth of the amoeba. On the other hand co-cultivation enhanced growth and survival of V. mimicus strains. Vibrio mimicus showed intracellular behaviour because bacteria were found to be localized in the cytoplasm of amoeba trophozoites and remain viable for 14 days. The cysts protected intracellular V. mimicus from high level of gentamicin. The intracellular growth of V. mimicus in A. castellanii suggests a role of A. castellanii as a host for V. mimicus.
PB  - Blackwell Publishing/Wiley
JF  - Environmental microbiology reports
VL  - 2
SN  - 1758-2229
TI  - Interaction between Vibrio mimicus and Acanthamoeba castellanii.
SP  - 166
AV  - restricted
EP  - 171
ER  -