creators_name: Abd, Hadi
creators_name: Valeru, Soni Priya
creators_name: Sami, Susan Marouf
creators_name: Saeed, Amir
creators_name: Raychaudhuri, Saumya
creators_name: Sandström, Gunnar
type: article
datestamp: 2012-02-28 16:07:47
lastmod: 2012-02-28 16:07:47
metadata_visibility: show
title: Interaction between Vibrio mimicus and Acanthamoeba castellanii.
ispublished: pub
subjects: QR
full_text_status: restricted
note: Copyright of this article belongs to Wiley.
abstract: Vibrio mimicus is a Gram-negative bacterium, which causes gastroenteritis and is closely related to Vibrio cholerae. The environmental reservoir of this bacterium is far from defined. Acanthamoeba as well as Vibrio species are found in diverse aquatic environments. The present study was aimed to investigate the ability of A. castellanii to host V. mimicus, the role of bacterial protease on interaction with A. castellanii and to disclose the ability of cysts to protect intracellular V. mimicus. Co-cultivation, viable counts, gentamicin assay, electron microscopy and statistical analysis showed that co-cultivation of wild type and luxO mutant of V. mimicus strains with A. castellanii did not inhibit growth of the amoeba. On the other hand co-cultivation enhanced growth and survival of V. mimicus strains. Vibrio mimicus showed intracellular behaviour because bacteria were found to be localized in the cytoplasm of amoeba trophozoites and remain viable for 14 days. The cysts protected intracellular V. mimicus from high level of gentamicin. The intracellular growth of V. mimicus in A. castellanii suggests a role of A. castellanii as a host for V. mimicus.
date: 2010-02
date_type: published
publication: Environmental microbiology reports
volume: 2
number: 1
publisher: Blackwell Publishing/Wiley
pagerange: 166-171
refereed: TRUE
issn: 1758-2229
official_url: http://onlinelibrary.wiley.com/doi/10.1111/j.1758-2229.2009.00129.x/abstract
citation:   Abd, Hadi and Valeru, Soni Priya and Sami, Susan Marouf and Saeed, Amir and Raychaudhuri, Saumya and Sandström, Gunnar  (2010) Interaction between Vibrio mimicus and Acanthamoeba castellanii.  Environmental microbiology reports, 2 (1).  pp. 166-171.  ISSN 1758-2229     
document_url: http://crdd.osdd.net/open/1116/1/saumya2010.pdf