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primary topic: Inhibition of Adipogenesis and Induction of Apoptosis and Lipolysis by Stem Bromelain in 3T3-L1 Adipocytes

Inhibition of Adipogenesis and Induction of Apoptosis and Lipolysis by Stem Bromelain in 3T3-L1 Adipocytes
type: AcademicArticle
type: Article
abstract: The phytotherapeutic protein stem bromelain (SBM) is used as an anti-obesity alternative medicine. We show at the cellular level that SBM irreversibly inhibits 3T3-L1 adipocyte differentiation by reducing adipogenic gene expression and induces apoptosis and lipolysis in mature adipocytes. At the molecular level, SBM suppressed adipogenesis by downregulating C/EBPα and PPARγ independent of C/EBPβ gene expression. Moreover, mRNA levels of adipocyte fatty acid-binding protein (ap2), fatty acid synthase (FAS), lipoprotein lipase (LPL), CD36, and acetyl-CoA carboxylase (ACC) were also downregulated by SBM. Additionally, SBM reduced adiponectin expression and secretion. SBM's ability to repress PPARγ expression seems to stem from its ability to inhibit Akt and augment the TNFα pathway. The Akt–TSC2–mTORC1 pathway has recently been described for PPARγ expression in adipocytes. In our experiments, TNFα upregulation compromised cell viability of mature adipocytes (via apoptosis) and induced lipolysis. Lipolytic response was evident by downregulation of anti-lipolytic genes perilipin, phosphodiestersae-3B (PDE3B), and GTP binding protein Giα1, as well as sustained expression of hormone sensitive lipase (HSL). These data indicate that SBM, together with all-trans retinoic-acid (atRA), may be a potent modulator of obesity by repressing the PPARγ-regulated adipogenesis pathway at all stages and by augmenting TNFα-induced lipolysis and apoptosis in mature adipocytes
date: 2012
volume: 7
issue: 1
is part of: PLoS ONE
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
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subject: QR Microbiology

Inhibition of Adipogenesis and Induction of Apoptosis and Lipolysis by Stem Bromelain in 3T3-L1 Adipocytes (PDF)
type: Document
license: http://creativecommons.org/licenses/by/3.0/
has file: pawan2012.pdf
has part: pawan2012.pdf

Public Library of Science (PLoS)
type: Organization

PLoS ONE
type: Collection
issn: 19326203

Naval Jit Kaur
type: Person
given name: Naval Jit
family name: Kaur

H Kitdorlang Dkhar
type: Person
given name: H Kitdorlang
family name: Dkhar

Ravikanth Nanduri
type: Person
given name: Ravikanth
family name: Nanduri

Massimo Federici
type: Person
given name: Massimo
family name: Federici

Pawan Gupta
type: Person
given name: Pawan
family name: Gupta

Ashwani Kumar
type: Person
given name: Ashwani
family name: Kumar

Sandeep Dave
type: Person
given name: Sandeep
family name: Dave

Inhibition of Adipogenesis and Induction of Apoptosis and Lipolysis by Stem Bromelain in 3T3-L1 Adipocytes (Indexer Terms)
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primary topic: Inhibition of Adipogenesis and Induction of Apoptosis and Lipolysis by Stem Bromelain in 3T3-L1 Adipocytes

QR Microbiology
type: Concept